Main Street in Louisville's downtown is one of the largest art districts in the city. It is one of the main stretches of the
First Friday Trolley Hop, a free, monthly event where the city's historic trolleys take participants to more than two dozen art galleries in the area. Some of Main Street's biggest attractions include:
- Louisville Slugger Field – The home of the Louisville Bats minor league baseball team.
- Actors Theatre of Louisville – An art gallery and performing arts venue best known for hosting the annual Humana Festival of New American Plays.
- The Kentucky Center for the Arts – An art gallery and performing arts venue hosting performances by the Louisville Ballet, Louisville Orchestra, Kentucky Opera, The Broadway Series, Stage One, and many other national and international acts.
- KentuckyShow! – A beautiful 32-minute long film about Kentucky shown in a 100-seat theater at the Kentucky Center.
- Muhammad Ali Center – A museum and center for education focused on presenting the life and values of Louisville-born Muhammad Ali.
- Kentucky Museum of Art and Craft – An art gallery that can host up to 200 artists at any one time created to support the arts and crafts community in Kentucky.
- 21C Museum Hotel – An upscale hotel, 9000-square-foot art museum, and fine-dining restaurant all in one that is one of Louisville's most interesting establishments.
- Louisville Science Center – An interactive science museum where kids can learn and experience science.
- Louisville Slugger Museum – A museum and factory where Louisville Slugger baseball bats are created and baseball's history is preserved. Visitors can tour the museum and watch as Louisville Slugger baseball bats are made.
- Frazier International History Museum – A 100,000-square-foot history museum dedicated to showcasing the history of the United States in its entirety.